Coding

Part:BBa_K3726029

Designed by: Santiago Barragán Ariza   Group: iGEM21_MADRID_UCM   (2021-10-06)


ppc_CDS

This part corresponds with the protein coding sequence for an Phosphoenolpyruvate carboxylase.

The codified enzyme from Corynebacterium glutamicum catalyzes the conversion of PEP to oxaloacetate via bicarbonate binding.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1099
    Illegal EcoRI site found at 1540
    Illegal PstI site found at 1684
    Illegal PstI site found at 1783
    Illegal PstI site found at 2368
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1099
    Illegal EcoRI site found at 1540
    Illegal PstI site found at 1684
    Illegal PstI site found at 1783
    Illegal PstI site found at 2368
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1099
    Illegal EcoRI site found at 1540
    Illegal BglII site found at 41
    Illegal BglII site found at 2081
    Illegal BamHI site found at 2179
    Illegal BamHI site found at 2194
    Illegal XhoI site found at 871
    Illegal XhoI site found at 1516
    Illegal XhoI site found at 1990
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1099
    Illegal EcoRI site found at 1540
    Illegal PstI site found at 1684
    Illegal PstI site found at 1783
    Illegal PstI site found at 2368
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1099
    Illegal EcoRI site found at 1540
    Illegal PstI site found at 1684
    Illegal PstI site found at 1783
    Illegal PstI site found at 2368
    Illegal NgoMIV site found at 1627
  • 1000
    COMPATIBLE WITH RFC[1000]


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